Present day practice An improved solubility test for haemoglobin
نویسنده
چکیده
Because conventional methods of testing for sickling are time consuming and liable to error other tests have been suggested for the detection of haemoglobin S (Hb S) (Ballard, Radel, Sakhadeo, and Schorr, 1970; Canning and Huntsman, 1970; Huntsman, Barclay, Canning, and Yawson, 1971; Cook and Raper, 1971). Most of these solubility tests for Hb S are adaptations of the quantitative precipitation methods introduced by Itano (1953) and Goldberg (1958). Published accounts leave doubt how well such tests behave in normal routine use. They are not suitable for testing samples from neonates since these may contain only a small proportion of Hb S. Occasionally they give false positive results due to heavy precipitates of plasma proteins. The volume of blood used is usually increased if the packed cell volume of the sample is low, in order to reduce the chance of a false negative result. This practice increases turbidity caused by precipitation of plasma proteins. These drawbacks are minimized in the method which follows.
منابع مشابه
Intra-erythrocytic haemoglobin crystals.
When investigating a newly discovered haemoglobin L, using Itano's solubility test (Itano, 1953), a resemblance to sickle cell haemoglobin was noticed inasmuch as solubility was not complete in 2.24 M phosphate buffer. This was later realized to be due to an artefact (see Ager and Lehmann, 1957), but at that time the cells containing haemoglobin L were tested for sickling by incubation with red...
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